精品蜜臀久久久久99网站,亚洲精品国产精华液,精品人妻无码专区在线视频,国产在线观看无码免费视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 02.03 人胰腺癌細(xì)胞

Panc 02.03 人胰腺癌細(xì)胞

簡要描述:CRL-2553 Panc 02.03 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1847

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

ATCC® Number: CRL-2553™ Price: $417.00

Designations: Panc 02.03

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 9,10

THO1: 6

TPOX: 9,12

vWA: 17

Age: 70 years

Gender: female

Ethnicity: White

Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 28.2 hrs [50655]

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2553

來自ATCC細(xì)胞,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測,確保細(xì)胞無污染,符合檢測合格標(biāo)準(zhǔn)。歡迎來詢價詳談

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美亚洲一区二区三区| 小sao货边洗澡边cao你| 国产在线无遮挡免费观看| 亚洲精品久久久久久久久久久| 与亲女洗澡时伦了视频| 女性女同性aⅴ免费观女性恋| 亚洲精品女同中文字幕| 人人妻人人澡人人爽欧美一区| 夜夜未满十八勿进的爽爽影院| 精品无码久久久久久久久| 精品人妻一区二区三区四区在线| 精品人妻一区二区三区四区在线| 免费又黄又爽又色的视频| 两个人看的www高清视频| 国产精品久久日日苍井空| 久久久久久久久久久国产| 无码八a片人妻少妇久久| 日日噜噜噜夜夜爽爽狠狠| 年级老师的滋味3中字视频| 99精品久久精品一区二区| 少妇av射精精品蜜桃专区| 40岁成熟女人牲交片20分钟| 欧美精品国产综合久久| 中文无码亚洲精品字幕| 日韩a片无码毛片免费看小说| 韩国三级中文字幕hd久久精品 | 中文字幕人妻无码一区二区三区 | 国产av国产精品白丝jk制服| 国产精品一区二区含羞草| 爆乳熟妇一区二区三区霸乳| 亚洲欧美国产毛片在线| 中文字幕丰满乱子伦无码专区| 一边啪啪一边呻吟av夜夜嗨| 国产又色又爽又高潮免费| 亚洲午夜福利在线观看| 男女野外做爰全过程69影院| 国产精品一区二区含羞草| 亚洲久热无码av中文字幕| 综合欧美五月丁香五月 | 亚洲人成影院在线无码按摩店| 欧美熟妇xxxxx欧美老妇不卡|